Applying one-molecule optical and AFM imaging and power-spectroscopy methods, we seek to unravel the mechanistic areas of the construction and dynamics of chromosomes. Our study pursuits incorporate:
Human Rad51 filaments on double- and one-stranded DNA: correlating typical and irregular sorts with recombination perform.
Multicolor imaging on the bacterial nucleoid and mobile division proteins with blue, orange, and around-infrared fluorescent proteins
Since 2000, Dekker has shifted the primary concentrate of his do the job to biophysics where by he experiments the Attributes of one biomolecules and cells utilizing the equipment of nanotechnology. This variation of discipline was driven by his fascination for the remarkable performing of Organic molecular constructions, along with through the lengthy-term standpoint a large number of exciting discoveries is usually predicted in this subject.[five] Current lines of research in his biophysics group are in the parts of:[six]
2015, discovery that condensin can be a very flexible protein framework; and very first detection of DNA knots employing nanopores
Direct immobilization of indigenous yeast iso-one cytochrome c on bare gold: quickly electron relay to redox enzymes and zeptomole protein-movie voltammetry
‘At first we looked at biological devices having a Bodily watch and acted on the basis in the instruments we realized. It took a few years in advance of we started Operating far more on the massive Organic issues. I then started to get https://www.ceesdekker.net in touch with it nanobiology, a expression that didn't exist at some time.
Finding out DNA loop extrusion by SMC proteins for chromosome architecture with single-molecule assays.
2003, demonstrated the first biosensors made outside of a carbon nanotube; solved the framework and mechanism of DNA fix proteins; and discovery of a brand new method for fabricating solid-point out nanopores for DNA translocation
one). The current–voltage curves confirmed considerable modulations with gate voltage, even at room temperature, which was according to band bending in the ~0.6 eV bandgap semiconducting nanotube. These details also showed which the conductance may very well be modulated by six orders of magnitude by switching the gate voltage. The outcome of this operate — a space-temperature transistor comprised of an individual carbon nanotube molecule — were released in Nature in May well 1998.
check out Share your interactive ePaper on all platforms and on your website with our embed purpose
Meanwhile, to ensure continued guidance, we're exhibiting the website with out types and JavaScript.
Publisher’s Observe Springer Character stays neutral with regard to jurisdictional promises in posted maps and institutional affiliations.
2004, discovery of recent physics in translocation of DNA by nanopores; very first experimental study of ions conduction in nanofluidic channels; initially electrochemistry with specific single-wall carbon nanotubes; STM detection and Charge of phonons in carbon nanotubes; initial electrical docking of microtubules on kinesin-coated nanostructures; to start with biophysics characterization on the mechanical Qualities of double-stranded RNA; and to start with solitary-molecule analyze of DNA translocation by a restriction-modification enzyme.